منابع مشابه
Fluorometric Assay of Tissue Histamine Kazuyoshi Kurahashi and Motohatsu Fujiwara
There have been numerous studies on the distribution of endogenous histamine (1-5). Many mammalian tissues contain large to small amounts of histamine preformed. Amongst them skin, stomach and lung contain extremely high concentration of histamine. These organs are exposed to the outside directly or indirectly and prone to traumatic injury. The tissue level of histamine has been estimated with ...
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To study allergic and immunologic incidence of secretory otitis media, histamine levels were investigated in 90 cases with middle ear effusion by means of the fluorometric assay . Histamine concentration in effusions were significantly higher than serum levels. Statistical analysis indicated that there was no differences between serous and mucoid effusions, nor between children and adults. Tymp...
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A sensitive fluorometric assay was developed for alcohol sulfotransferase (AST). This was the first continuous fluorometric assay reported for AST. It used 3'-phosphoadenosine 5'-phosphosulfate regenerated from 3-phosphoadenosine 5'-phosphate by a recombinant phenol sulfotransferase (PST) using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The recombinant PST did not use the alcohol...
متن کاملFluorometric assay for N-acetylprocainamide.
We describe a simple, rapid fluorometric assay for separate quantitative analysis of procainamide and N-acetylprocainamide in mixtures. The effective lenear range (fluorescence vs. concentration) in serum is 0.1 to 10.0 mg/liter, regardless of the ratio (by weight) of the two drugs from 1:10 to 10:1. Analytical recoveries by the extraction method used were 100.0 +/- 3.0% and 98.0 +/- 4.0%, resp...
متن کاملEarly detection of influenza virus by using a fluorometric assay of infected tissue culture.
A fluorometric substrate, 4-methylumbelliferyl-alpha-ketoside of N-acetylneuramide, was used directly on clinical specimens and infected tissue culture 24 h after inoculation for the detection of influenza viral neuraminidase. Viral neuraminidase was detected in infected tissue culture but not in clinical specimens. The sensitivity of the assay on tissue culture was 92%, and the specificity was...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1969
ISSN: 0021-5198
DOI: 10.1254/jjp.19.240